| Peer-Reviewed

Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus

Received: 27 August 2014     Accepted: 5 September 2014     Published: 20 September 2014
Views:       Downloads:
Abstract

Chemical extract of Nerium oleander (NO) is identified as in vitro antibacterial and antifungal activities. However, there is no any study about antiviral effect of NO distillate (NOD). The aim of this study was to evaluate in vitro effects of NOD alone and combined with acyclovir on Parainfluenza-3 (PI-3) virus. Lyophilized NOD was dissolved at concentration of 10 mg/mL with distillated water and filtered. The thiazolyl blue test was used to evaluate the cytotoxic activity of the NOD, and acyclovir. NOD did not cause cytotoxicity against to MDBK cells up to 10 mg/mL concentrations while acyclovir did not cause cytotoxicity up to 0.97 µg/mL. NOD (10 mg/mL), acyclovir (0.97 µg/mL) and NOD + acyclovir were treated with 100 TCID50 diluted PI-3 in 96-well plates. Antiviral activity of NOD was statistically (P<0.05) different from NOD + acyclovir against PI-3. The most effective results obtained from using single of NOD (P<0.05) while acyclovir was not effective (P>0.05). In conclusion, it is referred that NOD has antiviral effect and it can be evaluated in the treatment of PI-3 in future.

Published in Animal and Veterinary Sciences (Volume 2, Issue 5)
DOI 10.11648/j.avs.20140205.14
Page(s) 150-153
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

PI-3, Nerium Oleander, Acyclovir, Antiviral

References
[1] Turner, R.B. and Couch, R.B. (2007). Rhinoviruses: Parainfluenza Viruses: the viruses and their replication. In: Fields Virology. Eds. by Knipe, D.M. and Howley, P.M. 5th edition, Lippincott, Williams & Wilkins, Philadelphia, Pp. 1449-1496.
[2] Carriere, P.D., Maxie, M.G., Wilkie, B.N., Savan, M., Valli, V.E. and Johnson, J.A. (1983). Exposure of calves to aerosols of parainfluenza-3 virus and Pasteurella haemolytica. Canadian Journal of Comparative Medicine, 47: 422-432.
[3] Haanes, E.J., Guimond P., Wardley, R. and Wardley, R. (1997). The bovine parainfluenza virus type-3 (BPIV-3) hemagglutinin/neuraminidase glycoprotein expressed in baculovirus protects calves against experimental BPIV-3 challenge. Vaccine, 15: 730-738.
[4] Begum, S., Siddiqui, B.S., Sultana, R., Zia, A. and Suria, A. (1999). Bio-active cardenolides from the leaves of Nerium oleander. Phytochemistry, 50: 435-438.
[5] Bandara, V., Weinstein, S.A., White, J. and Eddleston, M.A. (2010). Review of the natural history, toxinology, diagnosis and clinical management of Nerium oleander (common oleander) and Thevetiaperuviana (yellow oleander) poisoning. Toxicon: Official Journal of the International Society on Toxinology, 56: 273-281.
[6] Argiropoulosa, A. and Rhizopouloua, S. (2013). Morphological features of petals of Nerium oleander. Plant Biosystems, 147: 638-644.
[7] Newman, R.A., Yang, P., Pawlus, A.D. and Block, K.I. (2008). Cardiac glycosides as novel cancer therapeutic agents. Molecular Interventions, 8: 36-43.
[8] Gupta, V. and Mittal, P. (2010). Phytochemical and pharmacological potential of Nerium Oleander. International Journal of Pharmaceutical Sciences and Research, 1: 21-27.
[9] Ozdemir, O., Ciftci, M.K. and Maden, M. (2011). Oleander poisoning in cattle. Eurasian Journal of Veterinary Science, 27: 73-76.
[10] Wang, X., Plomley, J.B., Newman, R.A., and Cisneros, A. (2000). LC/MS/MS analyses of an oleander extract for cancer treatment. Analytical Chemistry, 72: 3547-3552.
[11] Turan, N., Akgun-Dar, K., Kuruca, S.E., Kilicaslan-Ayna, T., Seyhan, V.G., Atasever, B., Mericli, F. and Carin, M. (2006). Cytotoxic effects of leaf, stem and root extracts of Nerium oleander on leukemia cell lines and role of the p-glycoprotein in this effect. Journal of Experimental Therapeutics & Oncology, 6: 31-38.
[12] Adome, R.O., Gachihi, J.W., Onegi, B., Tamale, J. and Apio, S.O. (2003). The cardiotonic effect of the crude ethanolic extract of Nerium oleander in the isolated guinea pig hearts. African Health Sciences, 3: 77-82.
[13] Hussain, M.A. and Gorsi, M.S. (2004). Antimicrobial activity of Nerium Oleander Linn. Asian Journal of Plant Science, 3: 177-180.
[14] Hadizadeh, I., Peivastegan, B. and Kolahi, M. (2009). Antifungal activity of nettle (Urticadiocia L.), colocynth (Citrulluscolocynthis L. Schrad), oleander (Neriumoleande L.) and konar (Ziziphusspina-christi L.) Extracts on plants pathogenic fungi. Pakistan Journal of Biological Sciences, 12: 58-63.
[15] Dik, B., Sayin, Z. and Corum, O. (2013). Investigation of antimicrobial effect of Nerium Oleander distillate. Eurasian Journal of Veterinary Science, 29: 150-152.
[16] Erturk, O., Zihni, D. and Ali, O.B. (2000). Antiviral activity of some plant extracts on the replication of Autographa californica nuclear polyhedrosis virus. Turkish Journal of Biology, 24: 833-844.
[17] Rajbhandari, M., Wegner, U., Jülich, M., Schöpke, T. and Mentel, R. (2001). Screening of Nepalese medicinal plants for antiviral activity. Journal of Ethnopharmacology, 74: 251-255.
[18] Baytop, T. (1984). Herbal medicine in Turkey. Istanbul University Publications, No: 3255, Istanbul, Turkey, p: 411.
[19] Bas, A.L., Demirci, S., Yazihan, N., Uney, K., and Kaya, E.E. (2012). Nerium oleander distillate improves fat and glucose metabolism in high-fat diet-fed streptozotocin-induced diabetic rats. International Journal of Endocrinology, Article ID: 947187, 10 pages.
[20] Dik, B., Uney, K., Ozdemir, O., Demirci, S., Yazihan, N.A. and Bas, A.L. (2012). Acute oral toxicity of Nerium oleander distillate in rats. Journal of Veterinary Pharmacology and Therapeutics, 35: 96.
[21] Kars, M.D., Kars, G., Gunduz, U., Uney, K. and Bas, A.L. (2011). Effect of Nerium Oleander Distillate on MCF-7 breast cancer cell lines. Abstracts/Current Opinion in Biotechnology, 22: 126.
[22] El-Shazly, M.M., El-Zayat, E.M. and Hermersdorfer, H. (2000). Insecticidal activity, mammalian cytotoxicity and mutagenicity of an ethanolic extract from Nerium oleander (Apocynaceae). The Annals of Applied Biology, 136: 153-157.
[23] Serota, F.T., Starr, S.E., Bryan, C.K., Koch, P.A., Plotkin, S.A. and August, C.S. (1982). Acyclovir treatment of herpes zoster infections: use in children undergoing bone marrow transplantation. Journal of the American Medical Association, 247: 2132-2135.
[24] Corey, L., Fife, K.H., Benedetti, J.K., Winter, C.A., Fahnlander, A., Connor, J.D., Hintz, M.A. and Holmes, K.K. (1983). Intravenous acyclovir for the treatment of primary genital herpes. Annals of Internal Medicine, 98: 914-921.
[25] McConkey, D.J., Lin, Y., Nutt, L.K., Ozel, H.Z. and Newman, R.A. (2000). Cardiac glycosides stimulate Ca+2 increases and apoptosis in androgen-independent, metastatic human prostate adenocarcinoma cells. Cancer Research, 60: 3807-3812.
[26] Frey, H.R. and Liess, B. (1971). Vermehrungs kinetik und verwendbarkeit eines stark zytopatogenen VD-MD virusstamnes für diagnostische untersuchungen mit der mikrotiter-method. Zentralblatt Fur Veterinarmedizin Reihe B, 18: 61-71.
[27] Safadi, F.F., Xu, J., Smock, S.L., Kanaan, R.A., Selim, A.H., Odgren, P.R., Jr. Marks, S.C., Owen, T.A. and Popoff, S.N. (2003). Expression of connective tissue growth factor in bone: its role in osteoblast proliferation and differentiation in vitro and bone formation in vivo. Journal of Cellular Physiology, 196: 51-62.
[28] Cetin, G. (2013). Handbook of sheep and goat. Billur Publisher, Konya, pp. 365-377.
[29] Manna, S.K., Sah, N.K., Newman, R.A., Cisneros, A. and Aggarwal, B.B. (2001). Oleandrin suppresses activation of nuclear transcription factor-B, activator protein-1, and c-Jun NH2-terminal kinase. Cancer Research, 60: 3838-3847.
[30] Ni, D., Madden, T.L., Johansen, M., Felix, E., Ho, D.H. and Newman, R.A. (2002). Murine pharmacokinetics and metabolism of oleandrin, a cytotoxic component of Nerium oleander. Journal of Experimental Therapeutics & Oncology, 2: 278-285.
[31] Barbosa, R.R., Fontenele-Neto, J.D. and Soto-Blanco, B. (2008). Toxicity in goats caused by oleander (Nerium oleander). Research in Veterinary Science, 85: 279-281.
Cite This Article
  • APA Style

    Oguzhan Avci, Burak Dik. (2014). Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus. Animal and Veterinary Sciences, 2(5), 150-153. https://doi.org/10.11648/j.avs.20140205.14

    Copy | Download

    ACS Style

    Oguzhan Avci; Burak Dik. Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus. Anim. Vet. Sci. 2014, 2(5), 150-153. doi: 10.11648/j.avs.20140205.14

    Copy | Download

    AMA Style

    Oguzhan Avci, Burak Dik. Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus. Anim Vet Sci. 2014;2(5):150-153. doi: 10.11648/j.avs.20140205.14

    Copy | Download

  • @article{10.11648/j.avs.20140205.14,
      author = {Oguzhan Avci and Burak Dik},
      title = {Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus},
      journal = {Animal and Veterinary Sciences},
      volume = {2},
      number = {5},
      pages = {150-153},
      doi = {10.11648/j.avs.20140205.14},
      url = {https://doi.org/10.11648/j.avs.20140205.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140205.14},
      abstract = {Chemical extract of Nerium oleander (NO) is identified as in vitro antibacterial and antifungal activities. However, there is no any study about antiviral effect of NO distillate (NOD). The aim of this study was to evaluate in vitro effects of NOD alone and combined with acyclovir on Parainfluenza-3 (PI-3) virus. Lyophilized NOD was dissolved at concentration of 10 mg/mL with distillated water and filtered. The thiazolyl blue test was used to evaluate the cytotoxic activity of the NOD, and acyclovir. NOD did not cause cytotoxicity against to MDBK cells up to 10 mg/mL concentrations while acyclovir did not cause cytotoxicity up to 0.97 µg/mL. NOD (10 mg/mL), acyclovir (0.97 µg/mL) and NOD + acyclovir were treated with 100 TCID50 diluted PI-3 in 96-well plates. Antiviral activity of NOD was statistically (P0.05). In conclusion, it is referred that NOD has antiviral effect and it can be evaluated in the treatment of PI-3 in future.},
     year = {2014}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Determination of in Vitro Antiviral Activity of Nerium Oleander Distillate against to Parainfluenza-3 Virus
    AU  - Oguzhan Avci
    AU  - Burak Dik
    Y1  - 2014/09/20
    PY  - 2014
    N1  - https://doi.org/10.11648/j.avs.20140205.14
    DO  - 10.11648/j.avs.20140205.14
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
    SP  - 150
    EP  - 153
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20140205.14
    AB  - Chemical extract of Nerium oleander (NO) is identified as in vitro antibacterial and antifungal activities. However, there is no any study about antiviral effect of NO distillate (NOD). The aim of this study was to evaluate in vitro effects of NOD alone and combined with acyclovir on Parainfluenza-3 (PI-3) virus. Lyophilized NOD was dissolved at concentration of 10 mg/mL with distillated water and filtered. The thiazolyl blue test was used to evaluate the cytotoxic activity of the NOD, and acyclovir. NOD did not cause cytotoxicity against to MDBK cells up to 10 mg/mL concentrations while acyclovir did not cause cytotoxicity up to 0.97 µg/mL. NOD (10 mg/mL), acyclovir (0.97 µg/mL) and NOD + acyclovir were treated with 100 TCID50 diluted PI-3 in 96-well plates. Antiviral activity of NOD was statistically (P0.05). In conclusion, it is referred that NOD has antiviral effect and it can be evaluated in the treatment of PI-3 in future.
    VL  - 2
    IS  - 5
    ER  - 

    Copy | Download

Author Information
  • Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, Konya, Turkey

  • Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Selcuk, Konya, Turkey

  • Sections