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Detection of Respiratory Viral Antigens in Cattle Lung Tissues by Direct ELISA

Received: 13 August 2014     Accepted: 28 August 2014     Published: 10 September 2014
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Abstract

Bovine Respiratory Disease is one of the most important diseases with serious financial losses for the cattle industry in worldwide. The aim of this study was to detect the associations between respiratory viruses; bovine herpes virus 1, bovine viral diarrhea virus, bovine respiratory syncytial virus, and Para influenza virus 3 statuses of a herd and bovine respiratory disease occurrence. Present study describes virological distribution of bovine respiratory viruses in non-vaccinated cattle (for mentioned infections) of Central Anatolia, Turkey. A total of 24 lung tissue samples were collected during the December 2012 to January 2013 from cattle that died after manifesting clinical signs of respiratory system. Samples were successfully homogenized. Tissue samples were analyzed for detecting antigens by commercially available direct ELISA kit. BRSV antigens were detected in lung tissues 4 out of 24 tested cattle with a percentage of 16.6%, whereas BHV-1, BVDV and PI-3 were not found. BRSV may be common reason of respiratory diseases in herds. It has been also offered advice about prevention of respiratory viral infection for health planning. In conclusion, existence of BRSV infection is still defined and may play an important role in the respiratoric viral infection of cattle.

Published in Animal and Veterinary Sciences (Volume 2, Issue 5)
DOI 10.11648/j.avs.20140205.13
Page(s) 146-149
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

BHV-1, BRSV, BVDV, PI-3, ELISA

References
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  • APA Style

    Oguzhan Avci, Sibel Yavru, Mehmet Ekik. (2014). Detection of Respiratory Viral Antigens in Cattle Lung Tissues by Direct ELISA. Animal and Veterinary Sciences, 2(5), 146-149. https://doi.org/10.11648/j.avs.20140205.13

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    ACS Style

    Oguzhan Avci; Sibel Yavru; Mehmet Ekik. Detection of Respiratory Viral Antigens in Cattle Lung Tissues by Direct ELISA. Anim. Vet. Sci. 2014, 2(5), 146-149. doi: 10.11648/j.avs.20140205.13

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    AMA Style

    Oguzhan Avci, Sibel Yavru, Mehmet Ekik. Detection of Respiratory Viral Antigens in Cattle Lung Tissues by Direct ELISA. Anim Vet Sci. 2014;2(5):146-149. doi: 10.11648/j.avs.20140205.13

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  • @article{10.11648/j.avs.20140205.13,
      author = {Oguzhan Avci and Sibel Yavru and Mehmet Ekik},
      title = {Detection of Respiratory Viral Antigens in Cattle Lung Tissues by Direct ELISA},
      journal = {Animal and Veterinary Sciences},
      volume = {2},
      number = {5},
      pages = {146-149},
      doi = {10.11648/j.avs.20140205.13},
      url = {https://doi.org/10.11648/j.avs.20140205.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140205.13},
      abstract = {Bovine Respiratory Disease is one of the most important diseases with serious financial losses for the cattle industry in worldwide. The aim of this study was to detect the associations between respiratory viruses; bovine herpes virus 1, bovine viral diarrhea virus, bovine respiratory syncytial virus, and Para influenza virus 3 statuses of a herd and bovine respiratory disease occurrence. Present study describes virological distribution of bovine respiratory viruses in non-vaccinated cattle (for mentioned infections) of Central Anatolia, Turkey. A total of 24 lung tissue samples were collected during the December 2012 to January 2013 from cattle that died after manifesting clinical signs of respiratory system. Samples were successfully homogenized. Tissue samples were analyzed for detecting antigens by commercially available direct ELISA kit. BRSV antigens were detected in lung tissues 4 out of 24 tested cattle with a percentage of 16.6%, whereas BHV-1, BVDV and PI-3 were not found. BRSV may be common reason of respiratory diseases in herds. It has been also offered advice about prevention of respiratory viral infection for health planning. In conclusion, existence of BRSV infection is still defined and may play an important role in the respiratoric viral infection of cattle.},
     year = {2014}
    }
    

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    T1  - Detection of Respiratory Viral Antigens in Cattle Lung Tissues by Direct ELISA
    AU  - Oguzhan Avci
    AU  - Sibel Yavru
    AU  - Mehmet Ekik
    Y1  - 2014/09/10
    PY  - 2014
    N1  - https://doi.org/10.11648/j.avs.20140205.13
    DO  - 10.11648/j.avs.20140205.13
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
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    EP  - 149
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20140205.13
    AB  - Bovine Respiratory Disease is one of the most important diseases with serious financial losses for the cattle industry in worldwide. The aim of this study was to detect the associations between respiratory viruses; bovine herpes virus 1, bovine viral diarrhea virus, bovine respiratory syncytial virus, and Para influenza virus 3 statuses of a herd and bovine respiratory disease occurrence. Present study describes virological distribution of bovine respiratory viruses in non-vaccinated cattle (for mentioned infections) of Central Anatolia, Turkey. A total of 24 lung tissue samples were collected during the December 2012 to January 2013 from cattle that died after manifesting clinical signs of respiratory system. Samples were successfully homogenized. Tissue samples were analyzed for detecting antigens by commercially available direct ELISA kit. BRSV antigens were detected in lung tissues 4 out of 24 tested cattle with a percentage of 16.6%, whereas BHV-1, BVDV and PI-3 were not found. BRSV may be common reason of respiratory diseases in herds. It has been also offered advice about prevention of respiratory viral infection for health planning. In conclusion, existence of BRSV infection is still defined and may play an important role in the respiratoric viral infection of cattle.
    VL  - 2
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    ER  - 

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Author Information
  • Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075, Konya, Turkey

  • Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075, Konya, Turkey

  • Virology Laboratory, Veterinary Control Institute, 42080, Konya, Turkey

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