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Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis

Received: 13 August 2014     Accepted: 20 August 2014     Published: 30 August 2014
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Abstract

The purpose of the present study was to evaluate the possible effects of Bovine Herpes virus-1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), and Bovine Herpes virus-4 (BHV-4) involving metritis in the selected unvaccinated dairy cattle herds in Afyon province of Turkey by serologically and virologically methods. A total of 63 dairy cattle with metritis were sampled in order to investigate the presence of BHV-1, BVDV and BHV-4 infections. The sera samples were tested for presence of antibodies to BHV-1, BVDV and BHV-4 using a commercially available indirect Enzyme Linked Immunosorbent Assay. Leukocyte samples were tested for presence of BVDV viral genome using Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR) for BHV-1 and BHV-4 viral genome. Detectable antibodies were detected in 6 (9.52%) of 63 against BVDV and 51 (80.95%) of 63 against BHV-4. Detectable antibodies were detected against BVDV from 6 (9.52%) of 63 sera samples and BHV-4 from 51 (80.95%) of 63. No antibodies against BHV-1 were detected as well as the results of RT-PCR for BVDV, and PCR for BHV-1 were all negative. Positive PCR results found BHV-4 genome from 8 (12.69%) of 63 leukocyte samples. Presence of BVDV and BHV-4 antibodies in unvaccinated animals indicates that these cattle had contracted infection. In conclusion, BHV-4 infections may play a direct or indirect role in causing bovine metritis; therefore their importance in the etiology of metritis and their economic impact needs further attention.

Published in Animal and Veterinary Sciences (Volume 2, Issue 5)
DOI 10.11648/j.avs.20140205.12
Page(s) 142-145
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

BHV-1, BVDV, BHV-4, ELISA, PCR

References
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  • APA Style

    Sibel Yavru, Oguzhan Avci, Mehmet Kale. (2014). Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis. Animal and Veterinary Sciences, 2(5), 142-145. https://doi.org/10.11648/j.avs.20140205.12

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    ACS Style

    Sibel Yavru; Oguzhan Avci; Mehmet Kale. Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis. Anim. Vet. Sci. 2014, 2(5), 142-145. doi: 10.11648/j.avs.20140205.12

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    AMA Style

    Sibel Yavru, Oguzhan Avci, Mehmet Kale. Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis. Anim Vet Sci. 2014;2(5):142-145. doi: 10.11648/j.avs.20140205.12

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  • @article{10.11648/j.avs.20140205.12,
      author = {Sibel Yavru and Oguzhan Avci and Mehmet Kale},
      title = {Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis},
      journal = {Animal and Veterinary Sciences},
      volume = {2},
      number = {5},
      pages = {142-145},
      doi = {10.11648/j.avs.20140205.12},
      url = {https://doi.org/10.11648/j.avs.20140205.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140205.12},
      abstract = {The purpose of the present study was to evaluate the possible effects of Bovine Herpes virus-1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), and Bovine Herpes virus-4 (BHV-4) involving metritis in the selected unvaccinated dairy cattle herds in Afyon province of Turkey by serologically and virologically methods. A total of 63 dairy cattle with metritis were sampled in order to investigate the presence of BHV-1, BVDV and BHV-4 infections. The sera samples were tested for presence of antibodies to BHV-1, BVDV and BHV-4 using a commercially available indirect Enzyme Linked Immunosorbent Assay. Leukocyte samples were tested for presence of BVDV viral genome using Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR) for BHV-1 and BHV-4 viral genome. Detectable antibodies were detected in 6 (9.52%) of 63 against BVDV and 51 (80.95%) of 63 against BHV-4. Detectable antibodies were detected against BVDV from 6 (9.52%) of 63 sera samples and BHV-4 from 51 (80.95%) of 63. No antibodies against BHV-1 were detected as well as the results of RT-PCR for BVDV, and PCR for BHV-1 were all negative. Positive PCR results found BHV-4 genome from 8 (12.69%) of 63 leukocyte samples. Presence of BVDV and BHV-4 antibodies in unvaccinated animals indicates that these cattle had contracted infection. In conclusion, BHV-4 infections may play a direct or indirect role in causing bovine metritis; therefore their importance in the etiology of metritis and their economic impact needs further attention.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Serologic and Virologic Investigation of BHV-1, BVDV and BHV-4 in Cattle with Metritis
    AU  - Sibel Yavru
    AU  - Oguzhan Avci
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    DO  - 10.11648/j.avs.20140205.12
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
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    EP  - 145
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20140205.12
    AB  - The purpose of the present study was to evaluate the possible effects of Bovine Herpes virus-1 (BHV-1), Bovine Viral Diarrhea Virus (BVDV), and Bovine Herpes virus-4 (BHV-4) involving metritis in the selected unvaccinated dairy cattle herds in Afyon province of Turkey by serologically and virologically methods. A total of 63 dairy cattle with metritis were sampled in order to investigate the presence of BHV-1, BVDV and BHV-4 infections. The sera samples were tested for presence of antibodies to BHV-1, BVDV and BHV-4 using a commercially available indirect Enzyme Linked Immunosorbent Assay. Leukocyte samples were tested for presence of BVDV viral genome using Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR) for BHV-1 and BHV-4 viral genome. Detectable antibodies were detected in 6 (9.52%) of 63 against BVDV and 51 (80.95%) of 63 against BHV-4. Detectable antibodies were detected against BVDV from 6 (9.52%) of 63 sera samples and BHV-4 from 51 (80.95%) of 63. No antibodies against BHV-1 were detected as well as the results of RT-PCR for BVDV, and PCR for BHV-1 were all negative. Positive PCR results found BHV-4 genome from 8 (12.69%) of 63 leukocyte samples. Presence of BVDV and BHV-4 antibodies in unvaccinated animals indicates that these cattle had contracted infection. In conclusion, BHV-4 infections may play a direct or indirect role in causing bovine metritis; therefore their importance in the etiology of metritis and their economic impact needs further attention.
    VL  - 2
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    ER  - 

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Author Information
  • Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, Turkey

  • Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, Turkey

  • Department of Virology, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, Burdur, 15100, Turkey

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