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Comparison Between Plethysmometer and Caliper Methods to Monitor Lesion-Size Induced by Leishmania major Infection in BALB/c Mouse Experimental Model

Received: 15 December 2015     Accepted: 25 December 2015     Published: 8 January 2016
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Abstract

In several parasitological diseases, as for leishmaniasis, measurement of the size of cutaneous lesions, which develop at the site of parasite inoculation in animal models, are the most commonly used index to assess disease progression, to compare parasites pathogenicity or virulence and to determine the effects of drug treatment and immunotherapies. The aim of this study is to compare the accuracy of two measurement tools i.e., the caliper and the plethysmometer to refine the lesion size determination. Our findings showed that the use of plethysmometer produced higher correlation with the importance of the lesion mass at experimental endpoints. These findings suggest that, for better differentiation in drug monitoring or Leishmania (L. ) strains’ virulence and pathogenicity, plethysmometer method is more sensitive to detect parasite-induced swelling and lesions differences at the end of experimental protocols when lesion size is important but caliper is more indicated for small lesions.

Published in Animal and Veterinary Sciences (Volume 3, Issue 6)
DOI 10.11648/j.avs.20150306.16
Page(s) 179-185
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Leishmania major, BALB/c Experimental Model, Lesion Size, Caliper, Plethysmometer

References
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  • APA Style

    Hanene Attia, Aymen Bali, Rabiaa M. Sghaier, Pablo A. Leon Martinez, Ghada Mkannez, et al. (2016). Comparison Between Plethysmometer and Caliper Methods to Monitor Lesion-Size Induced by Leishmania major Infection in BALB/c Mouse Experimental Model. Animal and Veterinary Sciences, 3(6), 179-185. https://doi.org/10.11648/j.avs.20150306.16

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    ACS Style

    Hanene Attia; Aymen Bali; Rabiaa M. Sghaier; Pablo A. Leon Martinez; Ghada Mkannez, et al. Comparison Between Plethysmometer and Caliper Methods to Monitor Lesion-Size Induced by Leishmania major Infection in BALB/c Mouse Experimental Model. Anim. Vet. Sci. 2016, 3(6), 179-185. doi: 10.11648/j.avs.20150306.16

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    AMA Style

    Hanene Attia, Aymen Bali, Rabiaa M. Sghaier, Pablo A. Leon Martinez, Ghada Mkannez, et al. Comparison Between Plethysmometer and Caliper Methods to Monitor Lesion-Size Induced by Leishmania major Infection in BALB/c Mouse Experimental Model. Anim Vet Sci. 2016;3(6):179-185. doi: 10.11648/j.avs.20150306.16

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  • @article{10.11648/j.avs.20150306.16,
      author = {Hanene Attia and Aymen Bali and Rabiaa M. Sghaier and Pablo A. Leon Martinez and Ghada Mkannez and Chiraz Atri and Khaled Chourabi and Fatma Z. Guerfali and Dhafer Laouini},
      title = {Comparison Between Plethysmometer and Caliper Methods to Monitor Lesion-Size Induced by Leishmania major Infection in BALB/c Mouse Experimental Model},
      journal = {Animal and Veterinary Sciences},
      volume = {3},
      number = {6},
      pages = {179-185},
      doi = {10.11648/j.avs.20150306.16},
      url = {https://doi.org/10.11648/j.avs.20150306.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150306.16},
      abstract = {In several parasitological diseases, as for leishmaniasis, measurement of the size of cutaneous lesions, which develop at the site of parasite inoculation in animal models, are the most commonly used index to assess disease progression, to compare parasites pathogenicity or virulence and to determine the effects of drug treatment and immunotherapies. The aim of this study is to compare the accuracy of two measurement tools i.e., the caliper and the plethysmometer to refine the lesion size determination. Our findings showed that the use of plethysmometer produced higher correlation with the importance of the lesion mass at experimental endpoints. These findings suggest that, for better differentiation in drug monitoring or Leishmania (L. ) strains’ virulence and pathogenicity, plethysmometer method is more sensitive to detect parasite-induced swelling and lesions differences at the end of experimental protocols when lesion size is important but caliper is more indicated for small lesions.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Comparison Between Plethysmometer and Caliper Methods to Monitor Lesion-Size Induced by Leishmania major Infection in BALB/c Mouse Experimental Model
    AU  - Hanene Attia
    AU  - Aymen Bali
    AU  - Rabiaa M. Sghaier
    AU  - Pablo A. Leon Martinez
    AU  - Ghada Mkannez
    AU  - Chiraz Atri
    AU  - Khaled Chourabi
    AU  - Fatma Z. Guerfali
    AU  - Dhafer Laouini
    Y1  - 2016/01/08
    PY  - 2016
    N1  - https://doi.org/10.11648/j.avs.20150306.16
    DO  - 10.11648/j.avs.20150306.16
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
    SP  - 179
    EP  - 185
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20150306.16
    AB  - In several parasitological diseases, as for leishmaniasis, measurement of the size of cutaneous lesions, which develop at the site of parasite inoculation in animal models, are the most commonly used index to assess disease progression, to compare parasites pathogenicity or virulence and to determine the effects of drug treatment and immunotherapies. The aim of this study is to compare the accuracy of two measurement tools i.e., the caliper and the plethysmometer to refine the lesion size determination. Our findings showed that the use of plethysmometer produced higher correlation with the importance of the lesion mass at experimental endpoints. These findings suggest that, for better differentiation in drug monitoring or Leishmania (L. ) strains’ virulence and pathogenicity, plethysmometer method is more sensitive to detect parasite-induced swelling and lesions differences at the end of experimental protocols when lesion size is important but caliper is more indicated for small lesions.
    VL  - 3
    IS  - 6
    ER  - 

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Author Information
  • Institut Pasteur de Tunis, LR11IPT02, Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), Tunis-Belvédère, Tunisia

  • Institut Pasteur de Tunis, LR11IPT02, Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), Tunis-Belvédère, Tunisia

  • Institut Pasteur de Tunis, LR11IPT02, Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), Tunis-Belvédère, Tunisia

  • Institut Pasteur de Tunis, LR11IPT02, Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), Tunis-Belvédère, Tunisia

  • Institut Pasteur de Tunis, LR11IPT02, Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), Tunis-Belvédère, Tunisia

  • Institut Pasteur de Tunis, LR11IPT02, Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), Tunis-Belvédère, Tunisia

  • Institut Pasteur de Tunis, LR11IPT02, Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), Tunis-Belvédère, Tunisia

  • Institut Pasteur de Tunis, LR11IPT02, Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), Tunis-Belvédère, Tunisia

  • Institut Pasteur de Tunis, LR11IPT02, Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), Tunis-Belvédère, Tunisia

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