| Peer-Reviewed

Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran

Received: 3 February 2015     Accepted: 11 February 2015     Published: 12 June 2015
Views:       Downloads:
Abstract

Infections by protozoan parasite Toxoplasma gondii are prevalent worldwide in animals and human. T. gondii is the causative agent of toxoplasmosis, one of the most prevalent parasitic infections to humans and domestic animals. If first be during pregnancy, T. gondii may be transferred vertically by tachyzoites that are passed to the embryo via the placenta. T. gondii may be transmitted horizontally in three phases of the life cycle, ingesting infectious oocysts from the environment or tissue cysts or tachyzoites which are contained in gastrointestinal of many different animals. Transmission may also occur via tachyzoites contained in blood products, texture transplants or non-pasteurized milk. Like rest of the world toxoplasmosis is prevalent in Iran. The present study aimed to determine T. gondii isolates from native cattle in Chaharmahal Va Bakhtiari province located in south west of Iran by molecular methods. In this study, 155 blood samples were collected from native cattle. Genomic DNA was extracted using DNA extraction Kit (Cinna Gen, Iran) according to the manufacturer protocol and PCR was performed using specific primers (ITS-F and ITS-R). Sixteen (6.95%) cattle were positive to T. gondii infection. The positive control samples showed the excepted amplification product specific for T. gondii (171 bp). Although the present results showed relatively low prevalence of T.gondii infection in Chaharmahal Va Bakhtiari native cattle, control and eradication programs seem to be still necessary to prevent the prevalence of this infection factor and economic losses.

Published in Animal and Veterinary Sciences (Volume 3, Issue 4)
DOI 10.11648/j.avs.20150304.11
Page(s) 102-105
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Toxoplasma gondii, Toxoplasmosis, Native Cattle, PCR, Iran

References
[1] Holec-Gąsior, L., Drapała, D., Dominiak-Górski, B. and Kur, J. (2013). Epidemiological study of Toxoplasma gondii infection among cattle in Northern Poland. Ann. Agric. Environ. Med, 20(4): 653-656.
[2] Haeri, M.R., Jalalizadegan, B., Tabatabaie, F. (2014). Recognition of Acute Toxoplasmosis with IgG Avidity ELISA Test in the Pregnant Women (The First Trimester) in Qom Province, Iran, during Two Years (2012-2013). A. J. L. S, 2(6-3): 18-21.
[3] Dubey, J.P., Rajendran, C., Ferreira, L.R., Martins, J., Kwok, O.C., Hill, D.E., Villena, I., Zhou, H., Su, C. and Jones, J.L. (2011). High prevalence and genotypes of Toxoplasma gondii isolated from goats, from a retail meat store, destined for human consumption in the USA. Int. J. Parasitology, 41(8): 827-833.
[4] Lopes, W.D., Rodriguez, J.D., Souza, F.A., dos Santos, T.R., dos Santos, R.S., Rosanese, W.M., Lopes, W.R., Sakamoto, C.A. and da Costa, A.J. (2013). Sexual transmission of Toxoplasma gondii in sheep. Vet Parasitol, 195(1-2): 47-56.
[5] Okada, T., Marmansari, D., Li, Z.M., Adilbish, A., Canko, S., Ueno, A., Shono, H., Furuoka, H. and Igarashi, M. (2013). A novel dense granule protein, GRA22, is involved in regulating parasite egress in Toxoplasma gondii. Mol Biochem Parasitol, 189(1-2): 5-13.
[6] Choi, K.M., Gang, J. and Yun, J. (2008). Anti-Toxoplasma gondii RH strain activity of herbal extracts used in traditional medicine. Int. J. Antimicrob Agents, 32(4): 360-362.
[7] Pratt, S., Wansadhipathi-Kannangara, N.K., Bruce, C.R., Mina, J.G., Shams-Eldin, H., Casas, J., Hanada, K., Schwarz, R.T., Sonda, S. and Denny, P.W. (2013). Sphingolipid synthesis and scavenging in the intracellular apicomplexan parasite, Toxoplasma gondii. Mol Biochem Parasitol, 187(1): 43-51.
[8] Grigg, M.E. and Sundar, N. (2009). Sexual recombination punctuated by outbreaks and clonal expansions predicts Toxoplasma gondii population genetics. Int .J. Parasitol, 39(8): 925-933.
[9] Masatani, T., Matsuo, T., Tanaka, T., Terkawi, M.A., Lee, E.G., Goo, Y.K., Aboge, G.O., Yamagishi, J., Hayashi, K., Kameyama, K., Cao, S., Nishikawa, Y. and Xuan, X. (2013). TgGRA23, a novel Toxoplasma gondii dense granule protein associated with the parasitophorous vacuole membrane and intravacuolar network. Parasitol Int, 62(4): 372-379.
[10] Miller, C.M., Boulter, N.R., Ikin, R.J. and Smith, N.C. (2009). The immunobiology of the innate response to Toxoplasma gondii. Int. J. Parasitol, 39(1): 23-39.
[11] Qu, D., Han, J. and Du, A. (2013). Enhancement of protective immune response to recombinant Toxoplasma gondii ROP18 antigen by ginsenoside Re. Exp Parasitol, 135(2): 234-239.
[12] Döşkaya, M., Caner, A., Ajzenberg D., Değirmenci, A., Dardé, M.L., Can, H., Erdoğan, D.D., Korkmaz, M., Uner, A., Güngör, C., Altıntaş, K. and Gürüz, Y. (2013). Isolation of Toxoplasma gondii strains similar to Africa 1 genotype in Turkey. Parasitol Int, 62(5): 471-474.
[13] Fallahi, S.h., Kazemi, B., Seyyed Tabaei, S.J., Bandehpour, M., Lasjerdi, Z., Taghipour, N., Zebardast, N., Nikmanesh, B., Omrani, V.F. and Ebrahimzadeh, F. (2014). Comparison of the RE and B1 gene for detection of Toxoplasma gondii infection in children with cancer. Parasitol Int, 63(1): 37-41.
[14] Rajaratnam, S. and Thiagarajan, T. (2012). Molecular characterization of wild mushroom. J. Exp. Bio, 2 (2): 369-373.
[15] Kent, T., Lapik, Y.R. and Pestov, D.G. (2009). The 5' external transcribed spacer in mouse ribosomal RNA contains two cleavage sites. RNA, 15(1): l14-120.
[16] Sevinsc, F. (2000). The seroprevalence of Toxoplasma gondii in goats detected by indirect hemagglutination (IHA) and indirect florescent antibody (IFA) tests in the region of Konya. Acta Parasitologica Turnica, 24(1): 57-80.
[17] Tavassoli, M., Ghorbanzadehghan, M. and Esmaeilnejad, B. (2013). Detection of Toxoplasma gondii in sheep and goats blood samples by PCR-RFLP in Urmia. Vet. Res. Forum, 4: 43–47.
[18] James, G.S., Sintchenko, V.G., Dickeson, D.J. and Gilbert, G.L. (1996). Comparison of cell culture, mouse inoculation, and PCR for detection of Toxoplasma gondii: effects of storage conditions on sensitivity. J. Clin. Microbiol, 34: 1572–1575.
[19] Mohammed Yesuf, K., Melese, Z.T. (2015). Prevalence of Toxoplasmosis in HIV/AIDS Patients in Mettu Karl Hospital. A. J. H. R, 3(3): 183-188.
[20] Ghazaei, C. (2006). Serological survey of antibodies to Toxoplasma gondii. Afr J Health Sci, 13(1-2): 131-4.
[21] Sharif, M., Gholami, Sh., Ziaei, H., Daryani, A., Laktarashi, B., Ziapour, S.P., Rafiei, A. and Vahedi, M. (2007). Seroprevalence of Toxoplasma gondii in cattle, sheep and goats slaughtered for food in Mazandaran province, Iran during. Vet. J, 174(2): 422-424.
[22] Nematollahi, A. and Moghadam, G. (2008). Survey on seroprevalence of anti-Toxoplasma gondii antibodies in cattle in Tabriz, Iran by IFAT. American. J. Anim. Vet. Sci, 3(1): 40-42.
[23] Raeghi, S., Akbarei, A. and Sadeghi, S. (2011). Seroprevalence of Toxoplasma gondii in sheep, cattle and horses in Urmia North-West of Iran. Iranian. J. Parasitol, 6(4): 90-94.
[24] Gilot-Fromont, E., Aubert, D., Belkilani, S., Hermitte, P., Gibout, O., Geers, R. and Villena, I. (2009). Villena, Landscape, herd management and within herd seroprevalence of Toxoplasma gondii in beef cattle herds from champagne-Ardenne, France. Vet Parasitol, 161: 36-40.
[25] González-Warleta, M., Castro-Hermida, J.A., Carro-Corral, C., Cortizo-Mella, J. and Mezo, M. (2008). Epidemiology of neosporosis in dairy cattle in Galicia (NW Spain). Parasitol Res 102(2): 243-249.
[26] Klun, I., Djurković-Djaković, O., Katić-Radivojević, S. and Nikolić, A. (2006). Cross-sectional survey on Toxoplasma gondii infection in cattle, sheep and pigs in Serbia: seroprevalence and risk factors. Vet Parasitol, 135(2): 121-131.
[27] Dubey, J.P. (1992). Isolation of Toxoplasma gondii from a naturally infected beef cow. J Parasitol, 78(1): 151-153.
[28] Huong, L.T., Ljungström, B.L., Uggla, A. and Björkman, C. (1998). Prevalence of antibodies to Neospora caninum and Toxoplasma gondii in cattle and water buffaloes in southern Vietnam. Vet Parasitol, 75(1): 53-57
[29] Fajardo, H.V., D'ávila, S., Bastos, R.R., Cyrino, C.D., de Lima Detoni, M., Garcia, J.L., das Neves, L.B., Nicolau, J.L. and Amendoeira, M.R. (2013). Seroprevalence and risk factors of toxoplasmosis in cattle from extensive and semi-intensive rearing systems at Zona da Mata, Minas Gerais state, Southern Brazil. Parasit Vectors, 6, 191.
[30] Dubey, J.P. (2009). Toxoplasmosis in sheep--the last 20 years. Vet Parasitol, 163(1-2): 1-14.
Cite This Article
  • APA Style

    Asghar Arshi, Esmaeil Mahmoudi, Mohammad-Saeid Jami, Abbas Doosti. (2015). Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran. Animal and Veterinary Sciences, 3(4), 102-105. https://doi.org/10.11648/j.avs.20150304.11

    Copy | Download

    ACS Style

    Asghar Arshi; Esmaeil Mahmoudi; Mohammad-Saeid Jami; Abbas Doosti. Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran. Anim. Vet. Sci. 2015, 3(4), 102-105. doi: 10.11648/j.avs.20150304.11

    Copy | Download

    AMA Style

    Asghar Arshi, Esmaeil Mahmoudi, Mohammad-Saeid Jami, Abbas Doosti. Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran. Anim Vet Sci. 2015;3(4):102-105. doi: 10.11648/j.avs.20150304.11

    Copy | Download

  • @article{10.11648/j.avs.20150304.11,
      author = {Asghar Arshi and Esmaeil Mahmoudi and Mohammad-Saeid Jami and Abbas Doosti},
      title = {Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran},
      journal = {Animal and Veterinary Sciences},
      volume = {3},
      number = {4},
      pages = {102-105},
      doi = {10.11648/j.avs.20150304.11},
      url = {https://doi.org/10.11648/j.avs.20150304.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150304.11},
      abstract = {Infections by protozoan parasite Toxoplasma gondii are prevalent worldwide in animals and human. T. gondii is the causative agent of toxoplasmosis, one of the most prevalent parasitic infections to humans and domestic animals. If first be during pregnancy, T. gondii may be transferred vertically by tachyzoites that are passed to the embryo via the placenta. T. gondii may be transmitted horizontally in three phases of the life cycle, ingesting infectious oocysts from the environment or tissue cysts or tachyzoites which are contained in gastrointestinal of many different animals. Transmission may also occur via tachyzoites contained in blood products, texture transplants or non-pasteurized milk. Like rest of the world toxoplasmosis is prevalent in Iran. The present study aimed to determine T. gondii isolates from native cattle in Chaharmahal Va Bakhtiari province located in south west of Iran by molecular methods. In this study, 155 blood samples were collected from native cattle. Genomic DNA was extracted using DNA extraction Kit (Cinna Gen, Iran) according to the manufacturer protocol and PCR was performed using specific primers (ITS-F and ITS-R). Sixteen (6.95%) cattle were positive to T. gondii infection. The positive control samples showed the excepted amplification product specific for T. gondii (171 bp). Although the present results showed relatively low prevalence of T.gondii infection in Chaharmahal Va Bakhtiari native cattle, control and eradication programs seem to be still necessary to prevent the prevalence of this infection factor and economic losses.},
     year = {2015}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran
    AU  - Asghar Arshi
    AU  - Esmaeil Mahmoudi
    AU  - Mohammad-Saeid Jami
    AU  - Abbas Doosti
    Y1  - 2015/06/12
    PY  - 2015
    N1  - https://doi.org/10.11648/j.avs.20150304.11
    DO  - 10.11648/j.avs.20150304.11
    T2  - Animal and Veterinary Sciences
    JF  - Animal and Veterinary Sciences
    JO  - Animal and Veterinary Sciences
    SP  - 102
    EP  - 105
    PB  - Science Publishing Group
    SN  - 2328-5850
    UR  - https://doi.org/10.11648/j.avs.20150304.11
    AB  - Infections by protozoan parasite Toxoplasma gondii are prevalent worldwide in animals and human. T. gondii is the causative agent of toxoplasmosis, one of the most prevalent parasitic infections to humans and domestic animals. If first be during pregnancy, T. gondii may be transferred vertically by tachyzoites that are passed to the embryo via the placenta. T. gondii may be transmitted horizontally in three phases of the life cycle, ingesting infectious oocysts from the environment or tissue cysts or tachyzoites which are contained in gastrointestinal of many different animals. Transmission may also occur via tachyzoites contained in blood products, texture transplants or non-pasteurized milk. Like rest of the world toxoplasmosis is prevalent in Iran. The present study aimed to determine T. gondii isolates from native cattle in Chaharmahal Va Bakhtiari province located in south west of Iran by molecular methods. In this study, 155 blood samples were collected from native cattle. Genomic DNA was extracted using DNA extraction Kit (Cinna Gen, Iran) according to the manufacturer protocol and PCR was performed using specific primers (ITS-F and ITS-R). Sixteen (6.95%) cattle were positive to T. gondii infection. The positive control samples showed the excepted amplification product specific for T. gondii (171 bp). Although the present results showed relatively low prevalence of T.gondii infection in Chaharmahal Va Bakhtiari native cattle, control and eradication programs seem to be still necessary to prevent the prevalence of this infection factor and economic losses.
    VL  - 3
    IS  - 4
    ER  - 

    Copy | Download

Author Information
  • Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

  • Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

  • Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

  • Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

  • Sections